epithelial sheets

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Date:Fri, 04 Jun 1999 13:39:29 -0600
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Kind friends,

I am posting this for someone else.  If you can help this person, please
respond to them directly as they apparently are not on histonet.  Tell them
how to subscribe to histonet while you are at it please.

I would like some help with staining problems.  I am removing epidermal
sheets from mouse skin using EDTA and then staining for Langerhans cells
(biotinylated anti-Ia primary antibody with ABC/DAB detection). I'm having
two kinds of problems:
1.	Cells in the center of the sheet don't stain.
2.	The epithelial sheets don't lie flat when I mount them on slides in

Can anyone give me some advice on how to correct these problems?  I would
really appreciate some help. 
Donna Kusewitt

Donna F. Kusewitt, DVM, PhD
Research Associate Professor
Department of Cell Biology and Physiology
Room 149 BMSB
915 Camino de Salud, NE
School of Medicine
University of New Mexico
Albuquerque, NM  87131
Telephone: 505-272-1302
FAX: 505-272-9105
Email: dkusewitt@salud.unm.edu <mailto:dkusewitt@salud.unm.edu> 

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