anti-CD31 and fixation

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Date:Fri, 04 Jun 1999 15:23:41 -0400

I was wondering if anyone out there finds anti-CD31 antibodies from Pharmingen 
(which are the only ones I'll use, they're great) are finicky in paraffin 
sections due to overfixing.  In other words, in my hands I find that even if 
the tissue is slightly overfixed I have a hard time with anti-CD31.  Any 
suggestions or comments?

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