Stain for Arsenic

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From:Roy Ellis <>
To:Histonet <>
Date:Wed, 02 Jun 1999 07:23:20 +0930
Content-Type:text/plain; charset="iso-8859-1"

Fix in 10% formalin containing 2.5% copper sulfate for 5 days. Wash for 24
hours in running water. Process and embed in parffin wax. Deparaffinized
sections show green granules of Scheele's green (CuHAsO3) which, though
insoluble in water, is dissolved by acids and by ammonium hydroxide. By
substituting copper acetate for the sulfate, the green granular paris green
or cupric acetoarsenite is produced. Its solubilities are similar (Castel's
method, Bull.Histol.Appliq. V13:106, 1936. A light safranin counterstain
gives good contrast. Method courtesy of Lillie, 3rd edition, Histopathologic
technic and practical histochemistry, page 445.
Roy Ellis

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