Re: water bath method for antigen retrieval.

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From:Jeff Silverman <>
To:Tom Wells <>, "'histonet'" <>
Date:Thu, 10 Jun 1999 18:29:52 -0400
Content-Type:text/plain; charset=ISO-8859-1

Jules Elias and Michelle Margiotta at Stony Brook published a
"kinder,gentler" on the breast section antigen retrieval for ER/PR- two
hours or more in an 80 degree C water bath. I'm sure other antibodies
respond similarly. Anyone?
Jeff Silverman 

> From: Tom Wells <>
> To: 'histonet' <>
> Subject: water bath method for antigen retrieval.
> Date: Wednesday, June 09, 1999 10:12 PM
> Does anyone use the water bath method for antigen retrieval? I know that
> Dako recommends it for their herceptest, but, does anyone use it for
> anything else?I am thinking of trying it out, but, since most water baths
> cost around $1000.00 I would like to get some feedback before I buy one.
> Thanks. Tom

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