Re: water bath method for antigen retrieval.
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| From: | Jeff Silverman <peptolab@hamptons.com> |
| To: | Tom Wells <tomers@home.com>, "'histonet'" <histonet@Pathology.swmed.edu> |
| Reply-To: | |
| Date: | Thu, 10 Jun 1999 18:29:52 -0400 |
| Content-Type: | text/plain; charset=ISO-8859-1 |
Tom,
Jules Elias and Michelle Margiotta at Stony Brook published a
"kinder,gentler" on the breast section antigen retrieval for ER/PR- two
hours or more in an 80 degree C water bath. I'm sure other antibodies
respond similarly. Anyone?
Jeff Silverman
----------
> From: Tom Wells <tomers@home.com>
> To: 'histonet' <histonet@pathology.swmed.edu>
> Subject: water bath method for antigen retrieval.
> Date: Wednesday, June 09, 1999 10:12 PM
>
> Does anyone use the water bath method for antigen retrieval? I know that
> Dako recommends it for their herceptest, but, does anyone use it for
> anything else?I am thinking of trying it out, but, since most water baths
> cost around $1000.00 I would like to get some feedback before I buy one.
> Thanks. Tom
>
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