Re: undecalcified bone

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From:Michelle M Sutorik <>
To:Gayle Callis <>
Date:Thu, 03 Jun 1999 14:27:15 -0400 (EDT)
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I was wondering if you could fax me a copy of the reference that you speak
of.  My fax # is 734-764-2469.  

Thank you so much,

Michelle M. Sutorik
Univ. Of MIch
Dept. or Oral Pathology/Oncology

On Thu, 3 Jun 1999, Gayle Callis wrote:

> Even if you put murine bone in polymethylmethacrylate, you will get 25%
> shrinkage, which is comparable to paraffin (have a publication on this,
> a comparison!)  Bone frozen sections are easiest done with an
> Instrumedic Cryojane Tape Transfer system on snap frozen bone and you will 
> need a tungsten carbide d profile knife.  Both are expensive, sectioning
> system is approx $7000 and knives from DDK are approx $950, not to mention
> a cryostat.  
> However, the results are well worth the investment, and saves a good deal
> of grief.
> Gayle Callis

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