Re: undecalcified bone
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From: | Michelle M Sutorik <istsmmmc@umich.edu> |
To: | Gayle Callis <uvsgc@msu.oscs.montana.edu> |
Reply-To: | |
Date: | Thu, 03 Jun 1999 14:27:15 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
Gayle,
I was wondering if you could fax me a copy of the reference that you speak
of. My fax # is 734-764-2469.
Thank you so much,
Michelle M. Sutorik
Univ. Of MIch
Dept. or Oral Pathology/Oncology
On Thu, 3 Jun 1999, Gayle Callis wrote:
> Even if you put murine bone in polymethylmethacrylate, you will get 25%
> shrinkage, which is comparable to paraffin (have a publication on this,
> a comparison!) Bone frozen sections are easiest done with an
> Instrumedic Cryojane Tape Transfer system on snap frozen bone and you will
> need a tungsten carbide d profile knife. Both are expensive, sectioning
> system is approx $7000 and knives from DDK are approx $950, not to mention
> a cryostat.
> However, the results are well worth the investment, and saves a good deal
> of grief.
>
> Gayle Callis
>
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