Re: undecalcified bone
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From: | Alan Bright <Bright@dial.pipex.com> |
To: | Gayle Callis <uvsgc@msu.oscs.montana.edu>, histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Thu, 03 Jun 1999 17:05:09 +0100 |
Content-Type: | text/plain; charset="iso-8859-1" |
I personally do not see the need for an expensive tape transfer system when
as a lot of you know, that with a durable cryostat fitted with a tungsten
carbide knife, the tape transfer is unnecessary, I know I am going to get
shot down for this, so can I have some back up please from all of you who
know this is correct.
Alan Bright
Bright Instrument Co.Ltd.
St Margarets Way
Huntingdon
PE18 6EB
England
Tel No; 01480 454528
Fax No;01480 456031
Email ; Bright@dial.pipex.com
-----Original Message-----
From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
Date: Thursday, June 03, 1999 04:09
Subject: undecalcified bone
>Even if you put murine bone in polymethylmethacrylate, you will get 25%
>shrinkage, which is comparable to paraffin (have a publication on this,
>a comparison!) Bone frozen sections are easiest done with an
>Instrumedic Cryojane Tape Transfer system on snap frozen bone and you will
>need a tungsten carbide d profile knife. Both are expensive, sectioning
>system is approx $7000 and knives from DDK are approx $950, not to mention
>a cryostat.
>However, the results are well worth the investment, and saves a good deal
>of grief.
>
>Gayle Callis
>
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