Re: decrease in iron in BM bx?
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From: | rschoonh@sph.unc.edu |
To: | fgallery@neurobio.sunysb.edu (Francie Gallery), HistoNet@Pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 15 Jun 1999 12:06:48 -0400 (Eastern Daylight Time) |
Content-Type: | TEXT/PLAIN; CHARSET=US-ASCII |
Francie,
Not supprising as most "decalcification" methods are actually
"demineralization" techniques and iron is a mineral. Suggest that you use a
chealating method using EDTA.
-- Begin original message --
> A question was posed to me this morning for the HistoNet folks.
>
> History:
> bone marrow biopsies fixed in 10% NBF, decalcified in 5% formic acid
> for 4-8hrs then (routine) processed in paraffin
> Observation:
> there is a marked decrease in iron in these BM bx specimens compared
> to that of the associated clots and aspirates (prepared as above except for
> the decal)
> Questions:
> 1) has anyone else experienced this (or similar) observation?
> 2) ideas for remedying this issue?
>
>
> Thanks in advance!
> ~Francie~
>
> FGALLERY@neurobio.sunysb.edu
>
> Department of Neurobiology & Behavior, SUNY at Stony Brook, Stony Brook, NY
> Histology Department, Suffolk County Medical Examiner's Office, Hauppauge,
> NY
>-- End original message --
best regards,
Bob
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
CB#7400
Chapel Hill, NC 27599
Phone
office 919-966-6343
Lab 919-966-6140
Fax 919-966-6123
**Suppose you were an idiot... And suppose you were a member of Congress ...
But I repeat myself.-Mark Twain**
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