Re: Used Automatic Stainer

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From:MICHAEL BECKER <msadk@worldnet.att.net>
To:HistoNet Server <HistoNet@Pathology.swmed.edu>
Reply-To:
Date:Sun, 13 Jun 1999 18:51:41 -0400
Content-Type:text/plain; charset=ISO-8859-1


Does anyone out there in HistoLand know of anyone or a company that wants
to sell or sells small, used (benchtop-say ~24 inches long) automatic
stainers.  I may be in the market soon and one estimate I received was
about $1650.00.  This would be for stat frozen sections-H&E and/or
Toliudine Blue stains.  I have a bench top fume hood already and we use
non-toxic solvents.  Any info would be greatly appreciated.  Thanks is
advance.

Sue Becker
Albany, NY  
----------
> From: HistoNet Server <HistoNet@Pathology.swmed.edu>
> To: HistoNet Server <HistoNet@pathology.swmed.edu>
> Subject: Daily Digest
> Date: Sunday, June 13, 1999 1:01 AM
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 07:30:40 -0500
> From: froyer@bitstream.net
> Subject: Re: water bath method for antigen retrieval.
> 
> Have you considered a refurbished (used) water bath for your application?
> Please contact me off the list for pricing and availability.
> 
> Ford Royer
> Analytical Instruments
> Minneapolis, MN
> (800) 565-1895
> email: froyer@bitstream.net
> Jeff Silverman wrote:
> 
> > Tom,
> > Jules Elias and Michelle Margiotta at Stony Brook published a
> > "kinder,gentler" on the breast section antigen retrieval for ER/PR- two
> > hours or more in an 80 degree C water bath. I'm sure other antibodies
> > respond similarly. Anyone?
> > Jeff Silverman
> >
> > ----------
> > > From: Tom Wells <tomers@home.com>
> > > To: 'histonet' <histonet@pathology.swmed.edu>
> > > Subject: water bath method for antigen retrieval.
> > > Date: Wednesday, June 09, 1999 10:12 PM
> > >
> > > Does anyone use the water bath method for antigen retrieval? I know
that
> > > Dako recommends it for their herceptest, but, does anyone use it for
> > > anything else?I am thinking of trying it out, but, since most water
baths
> > > cost around $1000.00 I would like to get some feedback before I buy
one.
> > > Thanks. Tom
> 
> 
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 09:45:32 -0500
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: buffy coat
> 
> Buffy coat - (buf'e) {earlier buffe, buffalo<OFr. Buffle< a dull brownish
> yellow.} a thin layer of white blood cells formed between the plasma and
the
> red cell layers following centrifugation of anticoagulated whole blood -
> appearing brownish yellow.
> 
> 
> Eric C. Kellar
> Histology/Immunohistochemistry
> University of Pittsburgh  Medical Center
> 
> 
> 
> 	----------
> 	From:  Garza-Williams, Sara [SMTP:Garza-Williams.Sara@tchden.org]
> 	Sent:  Friday, June 11, 1999 4:06 PM
> 	To:  'histonet@pathology.swmed.edu'
> 	Subject:  buffy coat
> 
> 	Hello everyone,
> 	Can someone share with me the origin of the word(s) buffy coat.
> 	A long story....
> 	thanks!!!
> 	Sara
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 11:00:20 -0500
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: Clorohydrate
> 
> CHLORAL HYDRATE
> 
> 2,2,2,-Trichloro-1,1,-ethanediol; First synthesized by Liebreich in 1869.
> Comprehensive description: J.E. Fairbrother in Analytical Profiles of
Drug
> Substances vol. 2, K. Florey, Ed.
> 
> THERAP CAT: Hypnotic; sedative, narcotic, anesthetic.
> 
> Substitutes : Xylene, toluene, chloroform, cedarwood oil or a mixture of
> equal parts of methyl salicylate (oil of wintergreen) and aniline
followed
> by pure methyl salicylate.
> 
> Remember Hunter S.Thompson in "Fear & Loathing in Las Vegas"? 
> We were somewhere around Barstow on the edge of the desert when the drugs
> began to take hold.
> And it only gets crazier and better: Suddenly there was a terrible roar
all
> around us and the sky was full of what looked like huge bats...
> 
> Thompson and his lawyer/companion were doing, among other things, ether. 
It
> wasn't ether as we know it that Rossetti* would
> have been doing but the chemical compound Chloral Hydrate (from which
ether
> and chloroform came from). It would have
> been prepared in an alcohol based tincture and was doctor prescribed.
> Sometimes called "the knock out drug," it was of
> course addictive.
> 
> *Dante Gabriel Rossetti was born in the same year as Jules Verne, another
> Utopian novelist like friend William Morris. Like
> Lord Byron, they loved the medievalism of Walter Scott's writing, and the
> image of another 'more natural' era, like the world in
> which Dante Alighieri (1265-1321) populated. 
> 
> Before moving permanently to England, the elder Rossetti worked as
composer
> Rossini's librettist. In England he taught and
> worked as a Dante scholar, marrying the much younger Frances Polidori,
niece
> of Dr. John Polidori (who later wrote The
> Vampyre). He was Lord Byron's companion during his exile from England in
> 1816.
> 
> He resumed his relationship with the earthy Fanny Cornforth; she'd been
the
> model for one of his moral paintings.
> His ether addiction worried even him. In a letter to his brother William,
> who referred sadly to these as Gabriel's "chloralized
> years," wrote that he hoped people wouldn't find out about his ether use.
He
> was afraid that then his art would be discredited.
> 
> On Easter Sunday, 1882, he died at the country house of a friend, where
he'd
> gone in yet another vain attempt to recover his
> health, which had been destroyed by the chloral as his wife's had been
> destroyed by laudanum (a tincture containing opium).
> 
> 
> Eric C. Kellar
> Histology/Immunohistochemistry
> University of Pittsburgh Medical Center
> 
> 
> 
> 
> 	----------
> 	From:  Schell, Maria G. [SMTP:MSchell@mc.utmck.edu]
> 	Sent:  Thursday, June 10, 1999 3:52 PM
> 	To:  'HistoNet Server'
> 	Subject:  Clorohydrate
> 
> 	Is there a substitute for clorohydrate ?
> 	I would like to use it to clear some plant tissue, but it is a
> contrlled
> 	substance and very hard to obtain. This was used years ago but I
> don't know
> 	if there is something new that works just as well.
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 11:45:29 -0500
> From: burch007@mc.duke.edu
> Subject: Re: TBS phone number
> 
> The new telephone number for TBS is:
> 
> 1-919-384-9393 voice
> 1-919-394-9595 fax
> 
> They recently moved to a new complex and their number was changed.
> 
> JB
> 
> _______________________ Reply Separator _______________________
> 
> Subject: TBS phone number
> Author:  kkdulany@UNMC.EDU at internet
> Date:    6/11/99 9:23 AM
> 
> Good morning fellow histotechs,
> Yesterday I received a catalog from Triangle Biomedical Sciences, Inc.
and I
> wrote out an order to them.  They however did not include a price list so
I
> tried to call them. The number on their catalog is 919-477-9283.  I have
tried
> several times and the operator always comes on and says that is not a
working
> number.  Do any of you have a different number to reach them?  The number
must
> be a mis-print or  maybe they had a hurricane and wiped out the phone
system.
> Help if you can please.
> Karen Dulany HTL (ASCP)
> Omaha, NE
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 12:15:32 -0500
> From: Angel92764@aol.com
> Subject: Re: Does anyone recognise? Yes.
> 
> I have a GREAT protocol for the modified trichrome one-step.  I hope you
like 
> it.  We think it is great.  
> 
> 
> 
> MODIFIED TRICHROME ONE-STEP:
> 
> Formalin fixed
> Paraffin embedded tissue
> Cut sections at 4 microns
> 
> 1.  Hydrate tissue to water
> 2.  Heat bouins solution in microwave for 20 sec. 
> 3.  Place slides in warm bouin's for 5 min. 
> 4.  Rinse in tap water until yellow fades (rinse gently for approx. 2
min.)
> 5.  Mix equal parts of Weigert's A&B (20 ml each) and place in a plastic
>       coplain jar. 
> 6.  Place slides in weigert's for 10 min.
> 7.  Rinse slides under LIGHTLY  RUNNING tap water for 10 min.
> 8.  Pour on and then off (quickly) with biebrich scarlett solution
(one-step  
>           
>       doesn't have enough red)
> 9.  Place in one-step solution for 23 min.  (we let it set at room temp.
to
>       slightly warm starting at the begining of the procedure so it will
have 
> time
>       to become room temp. as much as possible.)
> 10.  Rinse in 3 changes of deionized water
> 11.  Place in 0.5% acidic acid solution for 2 min. 
> 12.  Dehydrate, clear and mount.  
> 
> 
> Good luck.  I hope you like this.  
> 
> 										
> 	Jeanie Wade, H.T.(ASCP)
> 										
> 	Pathology Associates of Tyler
> 										
> 	Tyler, TX
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 15:30:40 -0500
> From: Mbbiocare@aol.com
> Subject: Re: Memory Loss
> 
> I was worried about memory loss, but after speaking to a number of
people, 
> some that are not Histotechs, as we get older our brains are so full of 
> information, we need to somehow install more RAM. The women who are
around 40 
> yrs, might also be starting menopause, however I know a male who has the
same 
> complaint.
> Marianne
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 16:45:47 -0500
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: Clorohydrate
> 
> On Thu, 10 Jun 1999, Schell, Maria G. wrote:
> 
> > Is there a substitute for clorohydrate ?
> > I would like to use it to clear some plant tissue, but it is a
contrlled
> > substance and very hard to obtain. This was used years ago but I don't
know
> > if there is something new that works just as well.
> 
>   I think you must mean chloral hydrate. For a water-soluble
>   substance to enhance transparency, how about glycerol? You can
>   transfer the specimen from alcohol or water to an alcohol-glycerol
>   mixture (less viscous, so it penetrates better than pure
>   glycerol) and then leave uncovered for the alcohol to evaporate
>   over the course of a few days. The refractive index of glycerol
>   (1.47) is a bit low.  For real transparency dehydrate to 100%
>   alcohol, then transfer the specimen to benzyl benzoate (RI 1.57;
>   almost odourless), methyl benzoate (1.51; unpleasant smell) or methyl
>   salicylate (1.54; strong wintergreen smell). These three esters
>   are all miscible with 100% alcohol, xylene, and resinous mounting
>   media. (Glycerol mixes with alcohol or water, not with the other
>   liquids.)
> 
>   Hope this helps. 
> 
>    John A. Kiernan,
>    Department of Anatomy & Cell Biology,
>    The University of Western Ontario,
>    LONDON,  Canada  N6A 5C1
> 
> 
> 
> 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 18:00:56 -0500
> From: "Hendry, Chris I" <HendryC@mar.dfo-mpo.gc.ca>
> Subject: shelf lives
> 
> I was wondering if anyone had information on the shelf lives of commonly
> used histological chemicals, stock solutions, working solutions, stains,
> etc. I think it would be a good reference for most people if such a list
> existed.  Thank you in advance.
> 
> > Chris Hendry
> > Graduate Student
> > University of New Brunswick/
> > Department of Fisheries and Oceans
> > Biological Station
> > St. Andrews, NB E0G 2X0 Canada
> > (506) 529-8854 Phone
> > (506) 529-5862 Fax
> > e-mail: hendryc@mar.dfo-mpo.gc.ca
> > URL: http://www.geocities.com/CapeCanaveral/Hall/9440
> >  
> > To steal ideas from one person is plagiarism; to steal from many is
> > research. 
> > 
> > 
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 18:10:20 -0500
> From: fetching@webtv.net (bonnie greer)
> Subject: Re: Memory Loss
> 
> 
> I worry about the xylene causing headaches and depression!!!!! what are
> the effects of 25 years of exposure? Does anyone really have the facts?
> Does anyone know about the muscle pain we all seem to have????
> 
> 
> 
> 
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> Date: Sat, 12 Jun 1999 16:21:03 -0400 (EDT)
> From: Mbbiocare@aol.com
> Subject: Re: Memory Loss
> To: ss336@yahoo.com, HistoNet@Pathology.swmed.edu
> Message-id: <101fe0ce.24941b2f@aol.com>
> MIME-version: 1.0
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> 
> I was worried about memory loss, but after speaking to a number of
people, 
> some that are not Histotechs, as we get older our brains are so full of 
> information, we need to somehow install more RAM. The women who are
around 40 
> yrs, might also be starting menopause, however I know a male who has the
same 
> complaint.
> Marianne
> 
> 
> - --WebTV-Mail-1503502706-7540--
> 
> 
> ----------------------------------------------------------------------
> 
> Date: 12 Jun 1999 19:30:41 -0500
> From: Gervaip@aol.com
> Subject: Re: Memory Loss
> 
> Bonnie, I get headaches from xylene the first few days I return from
being 
> gone from the lab for 3 weeks or more.   And all the aches and pains most
of 
> seem to have is due to the repetitive motion involved in our jobs and
lack of 
> knowledge of ergonomic postures, furniture and instrumentation.  And age
also 
> plays an important role in all these repetitive motion induced problems. 

> pearl
> 
> 
> Here are the messages received yesterday!



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