Re: Used Automatic Stainer
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From: | MICHAEL BECKER <msadk@worldnet.att.net> |
To: | HistoNet Server <HistoNet@Pathology.swmed.edu> |
Reply-To: | |
Date: | Sun, 13 Jun 1999 18:51:41 -0400 |
Content-Type: | text/plain; charset=ISO-8859-1 |
Does anyone out there in HistoLand know of anyone or a company that wants
to sell or sells small, used (benchtop-say ~24 inches long) automatic
stainers. I may be in the market soon and one estimate I received was
about $1650.00. This would be for stat frozen sections-H&E and/or
Toliudine Blue stains. I have a bench top fume hood already and we use
non-toxic solvents. Any info would be greatly appreciated. Thanks is
advance.
Sue Becker
Albany, NY
----------
> From: HistoNet Server <HistoNet@Pathology.swmed.edu>
> To: HistoNet Server <HistoNet@pathology.swmed.edu>
> Subject: Daily Digest
> Date: Sunday, June 13, 1999 1:01 AM
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 07:30:40 -0500
> From: froyer@bitstream.net
> Subject: Re: water bath method for antigen retrieval.
>
> Have you considered a refurbished (used) water bath for your application?
> Please contact me off the list for pricing and availability.
>
> Ford Royer
> Analytical Instruments
> Minneapolis, MN
> (800) 565-1895
> email: froyer@bitstream.net
> Jeff Silverman wrote:
>
> > Tom,
> > Jules Elias and Michelle Margiotta at Stony Brook published a
> > "kinder,gentler" on the breast section antigen retrieval for ER/PR- two
> > hours or more in an 80 degree C water bath. I'm sure other antibodies
> > respond similarly. Anyone?
> > Jeff Silverman
> >
> > ----------
> > > From: Tom Wells <tomers@home.com>
> > > To: 'histonet' <histonet@pathology.swmed.edu>
> > > Subject: water bath method for antigen retrieval.
> > > Date: Wednesday, June 09, 1999 10:12 PM
> > >
> > > Does anyone use the water bath method for antigen retrieval? I know
that
> > > Dako recommends it for their herceptest, but, does anyone use it for
> > > anything else?I am thinking of trying it out, but, since most water
baths
> > > cost around $1000.00 I would like to get some feedback before I buy
one.
> > > Thanks. Tom
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 09:45:32 -0500
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: buffy coat
>
> Buffy coat - (buf'e) {earlier buffe, buffalo<OFr. Buffle< a dull brownish
> yellow.} a thin layer of white blood cells formed between the plasma and
the
> red cell layers following centrifugation of anticoagulated whole blood -
> appearing brownish yellow.
>
>
> Eric C. Kellar
> Histology/Immunohistochemistry
> University of Pittsburgh Medical Center
>
>
>
> ----------
> From: Garza-Williams, Sara [SMTP:Garza-Williams.Sara@tchden.org]
> Sent: Friday, June 11, 1999 4:06 PM
> To: 'histonet@pathology.swmed.edu'
> Subject: buffy coat
>
> Hello everyone,
> Can someone share with me the origin of the word(s) buffy coat.
> A long story....
> thanks!!!
> Sara
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 11:00:20 -0500
> From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
> Subject: RE: Clorohydrate
>
> CHLORAL HYDRATE
>
> 2,2,2,-Trichloro-1,1,-ethanediol; First synthesized by Liebreich in 1869.
> Comprehensive description: J.E. Fairbrother in Analytical Profiles of
Drug
> Substances vol. 2, K. Florey, Ed.
>
> THERAP CAT: Hypnotic; sedative, narcotic, anesthetic.
>
> Substitutes : Xylene, toluene, chloroform, cedarwood oil or a mixture of
> equal parts of methyl salicylate (oil of wintergreen) and aniline
followed
> by pure methyl salicylate.
>
> Remember Hunter S.Thompson in "Fear & Loathing in Las Vegas"?
> We were somewhere around Barstow on the edge of the desert when the drugs
> began to take hold.
> And it only gets crazier and better: Suddenly there was a terrible roar
all
> around us and the sky was full of what looked like huge bats...
>
> Thompson and his lawyer/companion were doing, among other things, ether.
It
> wasn't ether as we know it that Rossetti* would
> have been doing but the chemical compound Chloral Hydrate (from which
ether
> and chloroform came from). It would have
> been prepared in an alcohol based tincture and was doctor prescribed.
> Sometimes called "the knock out drug," it was of
> course addictive.
>
> *Dante Gabriel Rossetti was born in the same year as Jules Verne, another
> Utopian novelist like friend William Morris. Like
> Lord Byron, they loved the medievalism of Walter Scott's writing, and the
> image of another 'more natural' era, like the world in
> which Dante Alighieri (1265-1321) populated.
>
> Before moving permanently to England, the elder Rossetti worked as
composer
> Rossini's librettist. In England he taught and
> worked as a Dante scholar, marrying the much younger Frances Polidori,
niece
> of Dr. John Polidori (who later wrote The
> Vampyre). He was Lord Byron's companion during his exile from England in
> 1816.
>
> He resumed his relationship with the earthy Fanny Cornforth; she'd been
the
> model for one of his moral paintings.
> His ether addiction worried even him. In a letter to his brother William,
> who referred sadly to these as Gabriel's "chloralized
> years," wrote that he hoped people wouldn't find out about his ether use.
He
> was afraid that then his art would be discredited.
>
> On Easter Sunday, 1882, he died at the country house of a friend, where
he'd
> gone in yet another vain attempt to recover his
> health, which had been destroyed by the chloral as his wife's had been
> destroyed by laudanum (a tincture containing opium).
>
>
> Eric C. Kellar
> Histology/Immunohistochemistry
> University of Pittsburgh Medical Center
>
>
>
>
> ----------
> From: Schell, Maria G. [SMTP:MSchell@mc.utmck.edu]
> Sent: Thursday, June 10, 1999 3:52 PM
> To: 'HistoNet Server'
> Subject: Clorohydrate
>
> Is there a substitute for clorohydrate ?
> I would like to use it to clear some plant tissue, but it is a
> contrlled
> substance and very hard to obtain. This was used years ago but I
> don't know
> if there is something new that works just as well.
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 11:45:29 -0500
> From: burch007@mc.duke.edu
> Subject: Re: TBS phone number
>
> The new telephone number for TBS is:
>
> 1-919-384-9393 voice
> 1-919-394-9595 fax
>
> They recently moved to a new complex and their number was changed.
>
> JB
>
> _______________________ Reply Separator _______________________
>
> Subject: TBS phone number
> Author: kkdulany@UNMC.EDU at internet
> Date: 6/11/99 9:23 AM
>
> Good morning fellow histotechs,
> Yesterday I received a catalog from Triangle Biomedical Sciences, Inc.
and I
> wrote out an order to them. They however did not include a price list so
I
> tried to call them. The number on their catalog is 919-477-9283. I have
tried
> several times and the operator always comes on and says that is not a
working
> number. Do any of you have a different number to reach them? The number
must
> be a mis-print or maybe they had a hurricane and wiped out the phone
system.
> Help if you can please.
> Karen Dulany HTL (ASCP)
> Omaha, NE
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 12:15:32 -0500
> From: Angel92764@aol.com
> Subject: Re: Does anyone recognise? Yes.
>
> I have a GREAT protocol for the modified trichrome one-step. I hope you
like
> it. We think it is great.
>
>
>
> MODIFIED TRICHROME ONE-STEP:
>
> Formalin fixed
> Paraffin embedded tissue
> Cut sections at 4 microns
>
> 1. Hydrate tissue to water
> 2. Heat bouins solution in microwave for 20 sec.
> 3. Place slides in warm bouin's for 5 min.
> 4. Rinse in tap water until yellow fades (rinse gently for approx. 2
min.)
> 5. Mix equal parts of Weigert's A&B (20 ml each) and place in a plastic
> coplain jar.
> 6. Place slides in weigert's for 10 min.
> 7. Rinse slides under LIGHTLY RUNNING tap water for 10 min.
> 8. Pour on and then off (quickly) with biebrich scarlett solution
(one-step
>
> doesn't have enough red)
> 9. Place in one-step solution for 23 min. (we let it set at room temp.
to
> slightly warm starting at the begining of the procedure so it will
have
> time
> to become room temp. as much as possible.)
> 10. Rinse in 3 changes of deionized water
> 11. Place in 0.5% acidic acid solution for 2 min.
> 12. Dehydrate, clear and mount.
>
>
> Good luck. I hope you like this.
>
>
> Jeanie Wade, H.T.(ASCP)
>
> Pathology Associates of Tyler
>
> Tyler, TX
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 15:30:40 -0500
> From: Mbbiocare@aol.com
> Subject: Re: Memory Loss
>
> I was worried about memory loss, but after speaking to a number of
people,
> some that are not Histotechs, as we get older our brains are so full of
> information, we need to somehow install more RAM. The women who are
around 40
> yrs, might also be starting menopause, however I know a male who has the
same
> complaint.
> Marianne
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 16:45:47 -0500
> From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
> Subject: Re: Clorohydrate
>
> On Thu, 10 Jun 1999, Schell, Maria G. wrote:
>
> > Is there a substitute for clorohydrate ?
> > I would like to use it to clear some plant tissue, but it is a
contrlled
> > substance and very hard to obtain. This was used years ago but I don't
know
> > if there is something new that works just as well.
>
> I think you must mean chloral hydrate. For a water-soluble
> substance to enhance transparency, how about glycerol? You can
> transfer the specimen from alcohol or water to an alcohol-glycerol
> mixture (less viscous, so it penetrates better than pure
> glycerol) and then leave uncovered for the alcohol to evaporate
> over the course of a few days. The refractive index of glycerol
> (1.47) is a bit low. For real transparency dehydrate to 100%
> alcohol, then transfer the specimen to benzyl benzoate (RI 1.57;
> almost odourless), methyl benzoate (1.51; unpleasant smell) or methyl
> salicylate (1.54; strong wintergreen smell). These three esters
> are all miscible with 100% alcohol, xylene, and resinous mounting
> media. (Glycerol mixes with alcohol or water, not with the other
> liquids.)
>
> Hope this helps.
>
> John A. Kiernan,
> Department of Anatomy & Cell Biology,
> The University of Western Ontario,
> LONDON, Canada N6A 5C1
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 18:00:56 -0500
> From: "Hendry, Chris I" <HendryC@mar.dfo-mpo.gc.ca>
> Subject: shelf lives
>
> I was wondering if anyone had information on the shelf lives of commonly
> used histological chemicals, stock solutions, working solutions, stains,
> etc. I think it would be a good reference for most people if such a list
> existed. Thank you in advance.
>
> > Chris Hendry
> > Graduate Student
> > University of New Brunswick/
> > Department of Fisheries and Oceans
> > Biological Station
> > St. Andrews, NB E0G 2X0 Canada
> > (506) 529-8854 Phone
> > (506) 529-5862 Fax
> > e-mail: hendryc@mar.dfo-mpo.gc.ca
> > URL: http://www.geocities.com/CapeCanaveral/Hall/9440
> >
> > To steal ideas from one person is plagiarism; to steal from many is
> > research.
> >
> >
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 18:10:20 -0500
> From: fetching@webtv.net (bonnie greer)
> Subject: Re: Memory Loss
>
>
> I worry about the xylene causing headaches and depression!!!!! what are
> the effects of 25 years of exposure? Does anyone really have the facts?
> Does anyone know about the muscle pain we all seem to have????
>
>
>
>
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> Date: Sat, 12 Jun 1999 16:21:03 -0400 (EDT)
> From: Mbbiocare@aol.com
> Subject: Re: Memory Loss
> To: ss336@yahoo.com, HistoNet@Pathology.swmed.edu
> Message-id: <101fe0ce.24941b2f@aol.com>
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>
> I was worried about memory loss, but after speaking to a number of
people,
> some that are not Histotechs, as we get older our brains are so full of
> information, we need to somehow install more RAM. The women who are
around 40
> yrs, might also be starting menopause, however I know a male who has the
same
> complaint.
> Marianne
>
>
> - --WebTV-Mail-1503502706-7540--
>
>
> ----------------------------------------------------------------------
>
> Date: 12 Jun 1999 19:30:41 -0500
> From: Gervaip@aol.com
> Subject: Re: Memory Loss
>
> Bonnie, I get headaches from xylene the first few days I return from
being
> gone from the lab for 3 weeks or more. And all the aches and pains most
of
> seem to have is due to the repetitive motion involved in our jobs and
lack of
> knowledge of ergonomic postures, furniture and instrumentation. And age
also
> plays an important role in all these repetitive motion induced problems.
> pearl
>
>
> Here are the messages received yesterday!
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