Re: Sectioning Nondecalcified Mouse Bone

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From:Karen S Pawlowski <>
To:Histo-Scientific Research Laboratory <>
Date:Thu, 03 Jun 1999 08:43:56 -0500 (CDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII


I have inadvertently processed and sectioned undecalcified mouse otic
capsule in Spurrs (I was told they had already been decalcified.)  I
managed to get it to cut with a glass knife and the section thickness was
normal (2-6 microns).  The bone looked a little rough, but I hadn't tried 
lengthening the inflitration time.  Like I said, I thought they had been
decalcified.  It might work for you, as long as you give the media enough
time to completely inflitrate the bone.

Karen Pawlowski, MS
Sr. Res. Assoc., UT Southwestern Med. Ctr
PhD candidate, UT Dallas

On Wed, 2 Jun 1999, Histo-Scientific Research Laboratory wrote:

> Histonetters,
> Any suggestions on how to section non-decalcified mouse (femur and sternum)
> bones?  The client does not want them decalcified because he is afraid he
> will ruin his statistical measurements.  Is vibratome sectioning our only
> choice?  Any comments would be appreciated.
> -Tom Galati
> Histo-Scientific Research Labs.
> (540)856-2222

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