On Thu, 10 Jun 1999, Mickie L. Johnson wrote:

> Hi Histonetters!
> Our pathologists want us to work up Inhibin antibody on paraffin sections
> and would like to find a source with probable working dilution for formalin
> fixed tissue.. Any help would be appreciated.  Vendors welcome too.  ;)
> Thanks in advance.
> 
> Mickie
> 
> Michael L. Johnson, HT/HTL(ASCP)
> Histology Supervisor
> Sacred Heart Medical Center
> W. 101 8th Avenue
> Spokane, Washington 99220
> (509) 626-4418
> FAX (509) 455-2052
> johnsom@shmc.org
> 
> 

Hello Micheal,
I use Inhibin from Serotec/Harlan in Indianapolis. Cat# MCA951S.
It works wonderfully with a pretreatment of citra buffer and used at a
1:50 dilution.  I've been using an ovary follicle as a positive control. I
also use Dako's LSAB2 kit for secondary and tertiary antibodies.  Any
questions, just e-mail back.

Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ