Re: Histo in Plastic
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From: | Mary Lou Norman <mlm11@cornell.edu> |
To: | Katie B <bresee98@yahoo.com> |
Reply-To: | |
Date: | Thu, 10 Jun 1999 10:08:25 -0400 |
Content-Type: | text/plain; charset="us-ascii" |
Katie,
This is my favorite: Apparently Lee got around!
Lee's Methylene Blue - Basic Fuchsin
1) Stock Methylene Blue
0.5 g m.b. (CI 52015)
400 mL Distilled water (DH2O)
2) Stock Basic Fuchsin
0.5 g b.f. (CI 42510)
400 mL DH2O
3) Stock phosphate buffer 0.1 M pH 6.8
49 Ml 0.2 M sodium phosphate dibasic anhydrous (28.4g Na2H2PO4 in
1000mL DH2O)
51 Ml 0.2 M sodium phosphate monobasic monohydrate (27.6g
NaH2PO4'H2O in 1000mL DH2O)
4) 95% ethyl alcohol
5) Working m.b.-b.f.
12.0 mL stock m.b.
12.0 mL stock b.f.
21.0 mL stock buffer
15.0 mL 95% alcohol (DISCARD AFTER USE)
Procedure:
1) Stain in working solution for 10 seconds
2) Rinse in DH2O
3) Dip in 95% quickly w/agitation to remove background staining
4) Rinse in water
5) Dry and mount
Results: Nuclei- blue
Cytoplasm, cilia, certain cell granules - shades of pink and red
Cartilage and most cell granules - blue to purple
Ref: Sorvall Instruction Manuel, Staining Procedures for Plastic Embedded
Tissue,
Dupont, Co. Intruments Productsa, Biomedical Div., July, 1979
Where I got this: Techniques in Plastic for Light Microscopy
NSH Region IV Symposium
6-25-83 Ann Harbor, Michigan
Alexandra N. Brady, M.S., MT/HTL (ASCP)
Robert Schoonhoven, HTL (ASCP)
Phew!!
At 12:57 09-06-1999 -0700, you wrote:
>Looking for a recipe for Methylene Blue-Basic Fuchsin stain to do in
>GMA embedded tissues. My boss tells me it is the "old" way to do
>something like an H+E in plastic.
>
>Thanks!
>
>===
>Catherine "Katie" Bresee Bennett
>Laboratory for Experimental Pathology
>Department of Veterinary Pathology
>Michigan State University
>
>e-mail: bresee98@yahoo.com
>_________________________________________________________
>Do You Yahoo!?
>Get your free @yahoo.com address at http://mail.yahoo.com
>
>
>
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