Re: Histo in Plastic/H&E stain

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From:Sarah Christo <>,
Date:Thu, 10 Jun 1999 14:57:49 -0500
Content-Type:text/plain; charset=US-ASCII

Hi Katie,
   We do H&E on GMA.  This is the procedure:
1. Gill's Hematoxylin 30-35 minutes
2.  Two changes of distilled water, 10 dips each change
3.  Scott's Tap water for 3 minutes.
4.  Repeat rinses as in step 2.
5.  Eosin Y/Phloxine B for 6-10 minutes.
6.  Distilled water, 10 dips or less.
7.  2 changes of Abs, 2 changes xylene, coverslip.
(may be able to let it air dry after step 6 and coverslip)
2.5 gm Eosin Y
0.5 gm Phloxine B
0.6 ml glacial acetic acid
500 ml distilled water
filter before use.

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
College Station, TX  77868-4458

>>> Katie B <> 06/09 2:57 PM >>>
Looking for a recipe for Methylene Blue-Basic Fuchsin stain to do in
GMA embedded tissues.  My boss tells me it is the "old" way to do
something like an H+E in plastic.


Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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