Re: Cytokeratin CAM 5.2
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From: | Dana Settembre <settembr@UMDNJ.EDU> |
To: | "Tapper, Sheila" <STapper@smdc.org> |
Reply-To: | |
Date: | Tue, 15 Jun 1999 08:18:33 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Tue, 15 Jun 1999, Tapper, Sheila wrote:
> I am wondering if someone could share their protocol for this antibody. We
> use the Ventana ES. Thanks for any information!
>
> Sheila Tapper
> Anatomic Pathology Team Leader
> SMDC Health System
> Duluth, MN
> Stapper@smdc.org <mailto:Stapper@smdc.org>
>
>
Sheila,
I frequently use Cytokeratin Cam 5.2 from Becton-Dickinson Cat# 349205
It is a predilute, but my Dako Autostainer allows me to dilute it further.
I dilute it with the Dako Antibody Diluent at 1:1. I don't pretreat at
all with this. I use Dako LSAB2 kit for the 2ndary and tertiary and have
used other kits in the past (Vector's ABC) they all work fine. Then
DAB. I don't know what a Ventana ES is. I guess that's your autostainer.
Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ
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