Re: Cytokeratin CAM 5.2

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From:Dana Settembre <settembr@UMDNJ.EDU>
To:"Tapper, Sheila" <>
Date:Tue, 15 Jun 1999 08:18:33 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Tue, 15 Jun 1999, Tapper, Sheila wrote:

> I am wondering if someone could share their protocol for this antibody.  We
> use the Ventana ES.  Thanks for any information!
> Sheila Tapper
> Anatomic Pathology Team Leader
> SMDC Health System
> Duluth, MN
> <> 

I frequently use Cytokeratin Cam 5.2 from Becton-Dickinson Cat# 349205
It is a predilute, but my Dako Autostainer allows me to dilute it further.
I dilute it with the Dako Antibody Diluent at 1:1.  I don't pretreat at
all with this.  I use Dako LSAB2 kit for the 2ndary and tertiary and have
used other kits in the past (Vector's ABC) they all work fine.   Then
DAB.  I don't know what a Ventana ES is.  I guess that's your autostainer.

Dana Settembre
Immunohistochemistry Lab
University Hospital
Newark, NJ

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