Re: Cell Proliferation

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From:Katie B <bresee98@yahoo.com>
To:histonet@Pathology.swmed.edu
Reply-To:
Date:Thu, 03 Jun 1999 05:57:29 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

I have done studies examining both PCNA levels or BrdU labeling. 
Personally, as the technician who does the data acquisition, I prefer
BrdU as the quantitation is easier and less subjective.  BrdU
quantitation is a yes/no answer.  PCNA can be present at varying levels
in the cell population being examined.  Thus, quantitation for PCNA is
more subjective as the intensity levels need to be graded.

However, I agree with a previous post that it depends on what you are
looking for.  If you want to know what cells are proliferating at a
specific timepoint, BrdU injections are preferred.  There are other
chemicals similar to BrdU (FrdU?) and if you wanted to do a
muli-labeling protocol, it can be done with these other DNA analogs.

-Katie Bennett

--- Fernando Capela e Silva <fcs@uevora.pt> wrote:
> What's the best way (and the vantages and disavantages)for the
> quantification of cell proliferation (in my case chondrocytes): PCNA
> or
> BrdU?
> 
> Sincerely.
> Fernando Capela e Silva
> Departamento de Biologia
> Universidade de Evora
> Apartado 94
> 7002-554 ...vora
> PORTUGAL
> 
> Telephone: +351-66-740 98 81
> Telefax: +351-66-711 231
> E-mail: fcs@uevora.pt
> http://home.dbio.uevora.pt/~fcs/FCAPELAeSILVA.html
>  
> 
> 



===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

e-mail: bresee98@yahoo.com
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