Re: Anti-Human ALK Protein

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From:Phyllis Davie <>
To:"Willis, Donna" <>,
Date:Fri, 04 Jun 1999 11:27:30 -0700
Content-Type:text/plain; charset="US-ASCII"

Regarding the ALK antibody from DAKO (catalog# M7195, clone: ALK-1).  We 
are using this successfully.  We use an anaplastic large cell lymphoma as 
a control.  We pretreat by microwaving for 3 5-minute sessions (15 
minutes total) in pH 10 Tris.  The primary antibody dilution is 1:25, and 
we are using Vector Elite ABC detection.  This has been working very 
nicely for us.
  Good Luck

Phyllis Davie
Clinical Laboratory Supervisor
PhenoPath Laboratories
Seattle, WA
(206)374-9009  phone
(206)374-9009  fax

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