Re: Anti-Human ALK Protein
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From: | Phyllis Davie <pdavie@phenopath.com> |
To: | "Willis, Donna" <DonnaWillis@hmhs.com>, histonet@Pathology.swmed.edu |
Reply-To: | |
Date: | Fri, 04 Jun 1999 11:27:30 -0700 |
Content-Type: | text/plain; charset="US-ASCII" |
Regarding the ALK antibody from DAKO (catalog# M7195, clone: ALK-1). We
are using this successfully. We use an anaplastic large cell lymphoma as
a control. We pretreat by microwaving for 3 5-minute sessions (15
minutes total) in pH 10 Tris. The primary antibody dilution is 1:25, and
we are using Vector Elite ABC detection. This has been working very
nicely for us.
Good Luck
Phyllis Davie
Clinical Laboratory Supervisor
PhenoPath Laboratories
Seattle, WA
pdavie@phenopath.com
(206)374-9009 phone
(206)374-9009 fax
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