I just would like to express my opinion for the CryoJane Tape Transfer
System.  I have purchased the system twice last several years when I started
working for biotechnology companies (one in TX and the other one in MD).  My
experience with the system has been wonderful.  People who were having
troubles with making frozen sections could start making beautiful frozen
(rat) undecalcified bone sections after a brief demonstration.  Many people
who don't have histology background use our cryostat at my current company.
I don't know how they can start making quality frozen sections without the
CryoJane Tape Transfer System.

Hiro Nitta, Ph.D.
Histology Supervisor/Research Scientist
Osiris Therapeutics, Inc.
Baltimore, MD 

> ----------
> From: 	Alan Bright[SMTP:Bright@dial.pipex.com]
> Sent: 	Thursday, June 03, 1999 12:05 PM
> To: 	Gayle Callis; histonet@pathology.swmed.edu
> Subject: 	Re: undecalcified bone
> 
> I personally do not see the need for an expensive tape transfer system
> when
> as a lot of you know, that with a durable cryostat fitted with a tungsten
> carbide knife,  the tape transfer is unnecessary, I know I am going to get
> shot down for this, so can I have some back up please from all of you who
> know this is correct.
> 
> Alan Bright
> 
> Bright Instrument Co.Ltd.
> St Margarets Way
> Huntingdon
> PE18 6EB
> England
> 
> Tel No; 01480 454528
> Fax No;01480 456031
> Email ; Bright@dial.pipex.com
> 
> 
> 
> 
> 
> -----Original Message-----
> From: Gayle Callis <uvsgc@msu.oscs.montana.edu>
> To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
> Date: Thursday, June 03, 1999 04:09
> Subject: undecalcified bone
> 
> 
> >Even if you put murine bone in polymethylmethacrylate, you will get 25%
> >shrinkage, which is comparable to paraffin (have a publication on this,
> >a comparison!)  Bone frozen sections are easiest done with an
> >Instrumedic Cryojane Tape Transfer system on snap frozen bone and you
> will
> >need a tungsten carbide d profile knife.  Both are expensive, sectioning
> >system is approx $7000 and knives from DDK are approx $950, not to
> mention
> >a cryostat.
> >However, the results are well worth the investment, and saves a good deal
> >of grief.
> >
> >Gayle Callis
> >
> 
>