IHC speaking this is a little tuff.  If you stained with collagen I it would
probably stain both the bone and collagen.  You could try collagen II, but
that will probably not stain the collagen or the bone, it should be positive
for cartilage only.  Collagen III will again stain everything.  Perhaps
osteocalcin and osteopontin will stain just the bone.  The U of Iowa
Hybridoma Bank sells a great osteopontin.  I guess that what I would try
first is IHC with osteocalcin and osteopontin antibodies.
Patsy Ruegg

		-----Original Message-----
		From:	Mary Vaughan
[mailto:mvaughan@sc3102.med.buffalo.edu]
		Sent:	Thursday, June 03, 1999 8:25 AM
		To:	HistoNet@Pathology.swmed.edu
		Subject:	collagen vs. bone

		Good Morning Histonetters,
		        I just got a call about this, and thought I'd throw
it on the net
		and see what we come up with...
		        It seems that this person is dealing with a tumor
that has
		unmineralized bone in the center. He said that he did a
trichrome on it, and
		of course the collagen stained as well as the "bone". He
wants a stain [or
		maybe Ab would be better] that will enable him to
differentiate the collagen
		from the unmineralized bone. Any suggestions ...??
						Best Regards,
						Mary Vaughan HT(ASCP)
						Roswell Park Cancer
Institute
						Pharmacology + Therapeutics
						Elm + Carlton Sts.  CDC-121
						Buffalo, NY 14263