RE: Storage of frozen sections
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From: | Patsy.Ruegg@UCHSC.edu |
To: | vandeplas@aurion.nl |
Reply-To: | |
Date: | Thu, 10 Jun 1999 09:03:42 -0600 |
Content-Type: | text/plain; charset="iso-8859-1" |
I prefer storing frozen sections unfixed. Cut and air dry for 1 hour and
then wrap individually in foil and store in plastic zip bags in a -70 d C
freezer. When you are ready to use them either take a jar of fixative to
the freezer with you and take them out individually and put directly into 4
d C fixative or take them out and put into a dessicator for 15 min. or so
until they come to room temp before unwrapping and fixing.
Patsy Ruegg
-----Original Message-----
From: vandeplas@aurion.nl [mailto:vandeplas@aurion.nl]
Sent: Wednesday, June 09, 1999 4:21 PM
To: Histonet@Pathology.swmed.edu
Subject: Re: Storage of frozen sections
Jennifer wrote:
Good Morning Histonetters! Does anyone have experience
with storage of
sectioned frozen tissue??? In the past I have only
sectioned tissue that I
would immunostain immediately. Currently my boss is
concerned with losing
tissue each time that I would re-trim a block; thus he
prefers that I section
approximately 20 slides at a time per block and store the
slides for subsequent
immunohistochemical staining (to be performed ASAP). I
consulted one book which
suggested that fixed slides could be stored at -70 C if
dessicated! Any
opinions or experience in this matter??? Thank you very
much in advance!
-----------------------------------------------
Dear Jennifer,
The following is not my answer, but a previous answer of one
of the fellow
histonetters to a similar question.
We have the best luck with the black plastic boxes (25 slide
capacity)
with a small nylon process bag or lens paper containing
silica gel to absorb
moisture when you bring sections back to room temperature.
We also
wrap tape around outside to seal box, this may be overkill,
but it
provides label. Avoid calcium chloride, anhydrous as a
substitute
for silica gel, Drierite (sp?) is also a problem since these
exfoliate fine
particles that float around, could deposit on sections.
silica gel stays intact
and is reused for a long time.
Store sections fixed, air dried, in -80C freezer, then bring
them
to room temperature BEFORE opening the lid, 20 - 30 minutes.
also stored slides in plastic slide mailers with snap lids,
wrapped
in foil, either in a plastic zip lock, or two mailers per
black slide
box with silica gel, you can remove one box and keep other
for another
day.
These are frozen sections, have stored unfixed frozens in
slide mailers with
success, but only for a few weeks. Fixed sections store for
longer times
Regards, Peter
========================================
Peter van de Plas
AURION
Costerweg 5
6702 AA Wageningen
The Netherlands
phone: (31)-317-497676
fax: (31)-317-415955
http://www.aurion.nl
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