Paul,

The best thing about working with glutaraldehyde-f ixed tissues for IHC,
like repeatedly banging your head against a brick wall, is that it feels so
good when you stop. I suggest you get new tissue fixed in buffered formalin.

Brett


Brett M. Connolly, Ph.D.
Senior Research Biologist
Dept. of Human Genetics
Merck Research Laboratories
WP26A-3000
PO Box 4
West Point, PA 19486
tel. 215-652-2501
FAX 215-652-2075

> ----------
> From: 	Paul Kwan[SMTP:paul.kwan@es.nemc.org]
> Sent: 	Tuesday, June 08, 1999 6:11 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	IHC on Glutaraldehyde Fixed Tissues
> 
> Hi,
> 
> I have tissues which were fixed in glutaraldehyde and then embedded in 
> paraffin.
> 
> Does anyone have experience in performing IHC on sections processed this
> way? 
> Routine methods (antigen retrieval with citrate buffer etc.) do not seem
> to 
> produce good results.
> 
> The tissue were originally designated for EM. 
> 
> Thanks,
> 
> PKwan in Boston
>