I am also interested in any info about the alk1 protein Ab. We are having a
great deal of trouble finding a suitable control, so have no idea if the Ab
is working well or not. I have heard from another user that it can be some
what capricious! :)
b

> -----Original Message-----
> From:	Dana Settembre [SMTP:settembr@UMDNJ.EDU]
> Sent:	Friday, June 04, 1999 12:50 PM
> To:	Willis, Donna
> Cc:	histonet@Pathology.swmed.edu
> Subject:	Re: Anti-Human ALK Protein
> 
> On Fri, 4 Jun 1999, Willis, Donna wrote:
> 
> > Those that have used the DAKO Anti-Human ALK Protein would you please
> answer
> > a couple of questions.
> > 
> > 1.  What control tissue are you using?
> > 
> > 2.  What type of detection are you using?
> > 
> > We are using Ventana Nexes instruments.  After talking to DAKO they
> > suggested using the High Intensity Detection.  Has anyone tried this?
> > 
> > Thanks,
> > Donna Willis
> > Histology Lab Manager
> > Harris Methodist Fort Worth, Texas
> > 817-878-5644 office
> > 817-878-5698 fax
> > email: donnawillis@hmhs.com 
> > 
> 
> Donna,
> I too am having trouble with this antibody and I am using the Dako
> Autostainer and all of Dako's reagents.  We're waiting to hear from the
> lab at Sloan-Kettering Memorial Hospital in New York City who supposedly
> have it going.  I'll let you know what happens.
> 
> Dana Settembre
> Immunohistochemistry Lab
> University Hospital
> Newark, NJ
>