Histonetters:

    Thanks for all of your comments and advice. I forgot to mention that my
undecalcified bone specimen includes a metal pin that will also need to be
sectioned.  Based on your helpful suggestions I am planning to fix the
femurs in 4% paraformaldehyde then dehydrate in Cellosolve (Ethylene
Glycol) and embed in LR white with a cold curing UV accelerator.  Hopefully
this work out, luckily I have some positive controls to try it on.

My only concern is whether or not it is ok to hold my tissues in 4%
paraformaldehyde until I am ready to embed.  Is there a better non-alcohol
solution to hold them in for extended periods of time (2-3 weeks)?  Thanks
again for all of your help!!

John/Pro


John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G161
Ann Arbor, MI 48109-0486
my office 734-936-1635
lab office 734-763-9674