Frozen Spleen

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From:jennifer.hoover@pharma.Novartis.com
To:Histonet@Pathology.swmed.edu
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Date:Mon, 14 Jun 1999 09:05:55 -0400
Content-Type:text/plain; charset=us-ascii

     Hi Histonetters! Just a quick question concerning frozen spleen tissue!  I
have no previous experience with frozen spleen for immunohistochemistry but
currently it's driving me crazy.  Could anyone suggest the optimal protocol for
handling spleen tissue including fixation before freezing, air drying, optimal
post-fixation, ect???  Many, many thanks in advance!!!!

Jennifer Hoover
Histologist
Transplantation Biology
Novartis Pharmaceuticals
Summit, NJ





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