CI Methods

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From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:"'HISTONET@PATHOLOGY.SWMED.EDU'" <>, "'Suder, Joanne'" <>
Date:Wed, 02 Jun 1999 13:03:48 -0400
Content-Type:text/plain; charset="iso-8859-1"

Hale was first to suggest the use of colloidal iron for the demonstration of
acid polysaccharides in 1946. Since that time many modifications of the
method have been used to stain acid mucopolysaccharides. Hale's method was
modified by Muller in 1955*, who substituted a solution known as
'ferrihydrozydsol' resulting in colloidal ferric hydroxide and named for him
- 'Muller's Colloidal Iron Oxide Solution'. This reagent became the standard
component of most CI methods. Further, Muller's method was modified by Mowry
(1958) it did not  stain nuclei, cytoplasm and tubular cells blue as did
Hale's original method. Hale also did not use Van Gieson solution as a
counterstain as Joyce Weems pointed out.

*Acta Histochem. (Jena) 2:68, 1955. 

Eric C. Kellar
University of Pittsburgh Medical Center

	From:  Suder, Joanne []
	Sent:  Wednesday, June 02, 1999 11:55 AM

	  What is the difference between Mowry's colloidal iron and Hales'
	iron. I have look in every Histology book and cannot find the
answer. Please

	Joanne C. Suder BA, HT (ASCP)
	Anatomic Pathology Supervisor
	Fox Chase Cancer Center

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