C-Kit (CD117) Antibody

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From:"Fessler, Jim" <jifess@pahosp.com>
To:"'Histonet@pathology.swmed.edu'" <Histonet@Pathology.swmed.edu>
Date:Tue, 15 Jun 1999 07:45:47 -0400

Jim Fessler from Pennsylvania Hospital in beautiful downtown Philadelphia
here.  I am trying to get the proto-oncogene c-kit  anitbody to work for me.
I am using Dako's polyclonal rabbit anti-human c-kit (CD117). I've tried
stainning paraffin-embedded sections with and without antigen retrieval
using various dilutions. I understand that the epitope seems to be quite
sensitive to long term fixation in formalin  and have avoided this.  If I
can get some help or suggestions, I'd sure be greatful.

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