| From: | Richard Cartun <Rcartun@harthosp.org> |
| To: | jifess@pahosp.com, HistoNet@Pathology.swmed.edu |
| Reply-To: | |
| Date: | Tue, 15 Jun 1999 09:43:03 -0400 |
| Content-Type: | text/plain; charset=US-ASCII |
We use DAKO's CD117 (c-Kit) pAb at a dilution of 1:200 for 30 minutes with EnVision+/HRP detection (also 30 minutes). Heat-induced epitope retrieval (HIER) is used for pretreatment. Try using a gastric stromal tumor for your positive control. See Am J Surg Path 23(4):377-389, 1999 (April) - "Interstitial Cells of Cajal as Precursors of Gastrointestinal Stromal Tumors". The antibody has worked very well in our laboratory. R. Cartun