C-Kit (CD117) Antibody -Reply

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From:Richard Cartun <Rcartun@harthosp.org>
To:jifess@pahosp.com, HistoNet@Pathology.swmed.edu
Date:Tue, 15 Jun 1999 09:43:03 -0400
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We use DAKO's CD117 (c-Kit) pAb at a dilution of 1:200 for 30 minutes with EnVision+/HRP detection (also 30 minutes).  Heat-induced epitope retrieval (HIER) is used for pretreatment.  Try using a gastric stromal tumor for your positive control.  See Am J Surg Path 23(4):377-389, 1999 (April) - "Interstitial Cells of Cajal as Precursors of Gastrointestinal Stromal Tumors".  The antibody has worked very well in our laboratory.

R. Cartun

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