Buying a Cryostat

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From:Masayuki Miyagishima <>
Date:Fri, 04 Jun 1999 07:14:37 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

Thank you very much for a lot of suggestion!
Many people recommend Leica 1850. InstrumedicsUs tape transfer system had
a good
reputation, too, but we did not have a chance to try it. We received three
demos from Leica, Zeis and Shandon. We tried them with AccuEdge disposable
blades.   We are now wondering whether we should go with Leica 1850 or
Microm 505EVP.  The things we like about Leica 1850 is; 1) we can use the
whole edge of the blade since the blade holder moves together.  This let
us change the position of the blade frequently to assure that we are
making most of the disposable blade.  The things we like about Micron
505EVP are; 1)Digital system let us know how many microns we cut. 2)Vacuum
system keeps the cryostat rather clean, and might help cutting better in
some tissue.
It seems that Microm 505EVP has everything Leica has, and we are almost
willing to pay an extra money for getting it, but here are something I
don't like about Microm.  1) The width of anti-rolling plate is narrow so
that it damages the
working area of the disposable blade, which makes us only able to move the
blade a few times per each blades. 2) With the bladeholder attached to the
vacuum system, the angle of the holder were not able to be adjusted to 0
degree.  This problem might be the reason why I experienced more rolling
up of the sliced tissue with Microm than with Leica.

I hope somebody will give me a good suggestion to overcome these possible
drawback of Microm.  Has anyone tried a magnetic blade with Microm?

Again, thank you very much for your help in advance.

Masayuki Miyagishima, MD
University of Pittsburgh
Department of Surgery

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