Anti-Ly-6G, CD45, CD8a, CD4 and paraffin sections?????

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From:AndreaH@imclone.com
To:HistoNet Server <HistoNet@Pathology.swmed.edu>
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Date:Wed, 09 Jun 1999 14:57:06 +0000 (GMT)
Content-Type:text/plain; charset=us-ascii


Does anyone know if any of the following antibodies can successfully stain
paraffin embedded sections?  I know the spec sheets say to use acetone
frozens, but I thought someone might have tried it anyway?

purified anti-mouse Ly-6G (Gr-1)
purified anti-mouse CD45R/B220
purified anti-mouse CD8a (Ly-2)
purified anti-mouse CD4 (L3T4)

And if not, does anyone know of a vendor that sells these antibodies that
do stain paraffin sections.

Thanks in advance.
Andrea





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