| From: | Richard Cartun <Rcartun@harthosp.org> |
| To: | DonnaWillis@hmhs.com, HistoNet@Pathology.swmed.edu |
| Reply-To: | |
| Date: | Fri, 04 Jun 1999 15:26:11 -0400 |
| Content-Type: | text/plain; charset=US-ASCII |
We use the DAKO mAB to ALK1 (M7195) routinely. Our positive control is a t(2;5)-positive, anaplastic large cell lymphoma (CD30 positive). We use the mAb at a dilution of 1:50 for a 30 minute incubation (1:500 overnight) following HIER. We have obtained spectacular immunoreactivity with both LSAB+ and EnVision+ detection from DAKO. R. Cartun