Re: Re: [Histonet] Double immunostaining with two same source 1stAntibody

From:"tf"



Thanks very much Igor.

I'd like to share the information to other people who are interested in this.

 
"www.jacksonimmuno.com 
Check out their web site-technical pages-staining with 2 Abs from same
sources. Their protocols are good and they do work
Luck
igor"


2008-07-14 



tf 



发件人: Igor Nasonkin 
发送时间: 2008-07-14  10:06:55 
收件人: tifei@foxmail.com 
抄送: 
主题: Re: [Histonet] Double immunostaining with two same source 1stAntibody 
 
Www.jacksonimmuno.com
Check out their web site-technical pages-staining with 2 Abs from same
sources. Their protocols are good and they do work
Luck
igor
On 7/13/08 10:02 PM, "tf"  wrote:
> Some one suggested a "citrate buffer" treatment before 2nd IHC.
> However he als suggested be careful about losing anti-gen in this step.
> 
> Actually I used this step for BrdU staining pretreatment before adding HCl to
> broken DNA helix (brdu can be taken by cell and synthsized into DNA).
> It seems that citrate buffer has more function than simply the antigen
> retrieval.
> 
> Can anyone comment on this further?
> 
> 
> 2008-07-14 
> 
> 
> 
> tf 
> 
> 
> 
> 发件人: Neil Fournier
> 发送时间: 2008-07-14  03:26:20
> 收件人: tifei@foxmail.com
> 抄送: 
> 主题: [Histonet] Double immunostaining with two same source 1st Antibody
>  
> I am extremely interested in the suggestions you receive. I would like to do a
> similar procedure. If possible, would you be able forward them to me.
> 
> Much appreciated,
> 
> Neil
> 
> Neil M. Fournier
> Department of Psychology
> University of Saskatchewan
> 9 Campus Drive
> Saskatoon, SK, Canada
> S7N 5A5
> 
> Phone:  306-966-4024
>     Fax:  306-966-6630
> _______________________________________________
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