Re: [Histonet] troubleshooting

From:Rene J Buesa

Usually it is caused by drying the sections before being completely drained and at high temperature (I am sending you an article on the subject under separate cover).
Keeping a "fresh" tissue in any of the ways you describe will lead to putrefaction and complete loss of cellular detail.The best way will be to snap freezing it.
René J.

--- On Thu, 7/3/08, Karla Arrington  wrote:

From: Karla Arrington 
Subject: [Histonet] troubleshooting
Date: Thursday, July 3, 2008, 11:05 AM

2 questions for you all. What causes tissue sections after H&E stained fixed
in formlain, to have nuclear bubbling? Is it under fixation, prolonged fixation?
Also what is the best way to store for a short time, fresh, unfixed tissue
either in a saline moistened gauze and refrigerated or just placing it in
physiological saline at room temperature.  I am troubleshooting some problems.

Karla Arrington

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