Hi- Check in to Nile Red or Nile blue if you do not mind doing fluorescent microscopy. It works well on saturated fats and is quite specific. good luck.
U of Washington
On Fri, 11 Jul 2008, Griffin-Reyes, Michelle A wrote:
> I am having some troubles finding a way to be able to stain saturated
> lipids in FFPE mouse tissues. Osmium tetroxide and oil red O stain well
> but they are only showing unsaturated lipids. My lab is looking to see
> if there is a difference in lipid content of treated mice vs. control
> mice. So far, we have seen very little variability in the amount of
> unsaturated lipids during quantification which leads us to believe there
> may be a difference in saturated fats (based off of human results that
> we have our mouse model based off of). Does anyone have any suggestions?
> Thanks so much for the help!
> Michelle A. Griffin-Reyes
> Comparative Pathology Laboratory
> University of Iowa: College of Medicine
> Notice: This UI Health Care e-mail (including attachments) is covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, is confidential and may be legally privileged. If you are not the intended recipient, you are hereby notified that any retention, dissemination, distribution, or copying of this communication is strictly prohibited. Please reply to the sender that you have received the message in error, then delete it. Thank you.
> Histonet mailing list
Judith Williams, PhD, HT(ASCP)
Department of Comparative Medicine
University of Washington
Seattle, WA 98195
Histonet mailing list