Yes, we do this often.  You need to make sure that you can differentiate between the two reactivities (e.g., cytoplasmic vs. nuclear) if you have any permanent reactivity (DAB) from the first antibody.  Alternatively, you could use a different chromogen for the second antibody.  Yes, you will need to retrieve again.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Christine I. Braaten"  07/16/08 9:11 AM >>>
Good morning histonet subscribers! I have a question about restaining
previously stained slides for IHC. I know I have seen this before but
cannot access the archives. If I have already stained a slide for NSE
can I destain it and stain it with a different antibody? They have
different retrievals so should I retrieve again? Thanks in advance for
your replies. Christine


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