Re: [Histonet] Restaining IHC slides

From:"Richard Cartun"

Yes, we do this often.  You need to make sure that you can differentiate between the two reactivities (e.g., cytoplasmic vs. nuclear) if you have any permanent reactivity (DAB) from the first antibody.  Alternatively, you could use a different chromogen for the second antibody.  Yes, you will need to retrieve again.


Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Christine I. Braaten"  07/16/08 9:11 AM >>>
Good morning histonet subscribers! I have a question about restaining
previously stained slides for IHC. I know I have seen this before but
cannot access the archives. If I have already stained a slide for NSE
can I destain it and stain it with a different antibody? They have
different retrievals so should I retrieve again? Thanks in advance for
your replies. Christine

CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information.  Any unauthorized review, use, disclosure or distribution is prohibited.  If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message.
Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>