Amy:
Regardless of somebody or nobody doing that, it is a good idea and should be done.
The only thing is that you have to have a reference point that should be a KNOWN concentration reacted with the stain to refer to your determinations. Ideally you should have a low and high concentrations and determine a correlation, in the same way as with a colorimetric determination of the concentration of some substance with a colored reaction.
René J.

--- On Mon, 6/30/08, Amy Lee  wrote:

From: Amy Lee 
Subject: [Histonet] Quantification for Sirius red staining
To: "histonet" 
Date: Monday, June 30, 2008, 7:30 PM

Hello histonetters,
 
I've asked a few questions here and got many big help. I appreciated all
help you guys provided.
 
I have another question. I've done sirius red stain on paraffin sections
and need quantify it. I have image pro plus software. So I am thinking use it
to measure sirius red density. I am trying to find reference that other people
done that before (to support my idea).  Have you or anybody you know done
that before? I googled but not many helpful info availble.
 
Thanks for your help!
 
Amy



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