Re: [Histonet] PAX-2

From:Patti Loykasek

Hi Thomas. Zymed has a Pax-2 concentrate, I don't know if they have a
pre-dilute available. We use it on paraffin embedded human tissue with a
heat citrate pretreatment. I always think it best to determine your own
optimal titer - so variable depending on fixation, processing,  
detection, etc.... We always start with the manufacturers  
recommendation &
do a 'sandwich titer'. For example, if recommended is 1:100, we do 1:50,
1:100, & 1:250. Hope this helps.

Patti Loykasek BS, HTL, QIHC
PhenoPath Laboratories
Seattle, WA

> Hey there folks,
> Anyone running PAX-2?  I've had no luck finding it as a pre-dilute and
> have only found one vendor with a concentrate.  So...anyone know of a
> pre-dilute out there and are you running it?  Or if you're running  
> it as
> a concentrate, what is the titration?  Human clinical laboratory
> application is what I'm interested in.  Thanks in advance for any
> assistance here.
> Thomas Jasper HT (ASCP) BAS
> Histology Supervisor
> Central Oregon Regional Pathology Services
> Bend, OR 97701
> 541/693-2677
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