Re: [Histonet] IBF and prostates-your experience

From:Rene J Buesa



Probably, because in 3 hours in IBF the biopsies will probably be fixed with the alcohols contained in IBF (methanol and 2-propanol). What I don't see is why you want to transferáthem áto 10% NBF.
RenÚ J.

--- On Fri, 7/18/08, Greg Dobbin  wrote:

From: Greg Dobbin 
Subject: [Histonet] IBF and prostates-your experience
To: Histonet@lists.utsouthwestern.edu
Date: Friday, July 18, 2008, 10:24 AM

Hi folks,
If were to use IBF for the collection of prostate cores and they only
fixed in IBF for 3 hrs before being transferred to 10% formalin for
processing with everything else, are the benefits of better nuclear
morphology still likely to be realized? 
Thank you.
Greg

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

"Success is not the key to happiness. 
Happiness is the key to success. 
If you love what you are doing, 
you will be successful." 
- Albert Schweitzer


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