Some one suggested a "citrate buffer" treatment before 2nd IHC.
However he als suggested be careful about losing anti-gen in this step.

Actually I used this step for BrdU staining pretreatment before adding HCl to broken DNA helix (brdu can be taken by cell and synthsized into DNA).
It seems that citrate buffer has more function than simply the antigen retrieval.

Can anyone comment on this further?


2008-07-14 



tf 



发件人： Neil Fournier 
发送时间： 2008-07-14  03:26:20 
收件人： tifei@foxmail.com 
抄送： 
主题： [Histonet] Double immunostaining with two same source 1st Antibody 
 
I am extremely interested in the suggestions you receive. I would like to do a similar procedure. If possible, would you be able forward them to me.

Much appreciated, 

Neil

Neil M. Fournier
Department of Psychology
University of Saskatchewan
9 Campus Drive
Saskatoon, SK, Canada
S7N 5A5

Phone:  306-966-4024
    Fax:  306-966-6630