Just to clarify exactly what you are doing - are you using a rat antiMouse 
CD4 conjugated to Alexa 488?  After I get the answer, I will elaborate.  We 
do successful CD4 immunofluorescent staining but never use a CD4 primary 
direct, with the primary conjugated to the fluorphore.    We have four 
different ways of doing the staining.

Gayle M. Callis
HTL/HT/MT(ASCP)
Bozeman MT



----- Original Message ----- 
From: "McMahon, Loralee A" 
To: 
Sent: Thursday, July 17, 2008 9:38 AM
Subject: [Histonet] CD4 Fluorescent Staining


I am trying an antibody called Alexa Fluor 488 anti-mouse CD4 on fresh 
frozen mouse tissue.  Having only worked with AED/DAB staining, not very 
familiar with this.
Does anyone have any experience with this and if you could send me some 
tips/protocols.  Thanks ahead of time


Loralee McMahon, HTL (ASCP)
ICC Supervisor
University of Rochester
Department of Pathology

(585) 275-7210

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