Try this protocol and adjust to your lab. See how the microtomy is for
you.
Rats, Dog,Primate,Pig all gets processed on the same protocol. 40 min in
all solutions +parrafin.(Formalin, 70, 80,95,95,100,100,100, xylene 3
changes). Including eyes. Dog &primate eyes are fixed in 3%
gluteraldehdye then transferred to 10%formalin.
All Testes and Rodent eyes start in 70% alcohol,80,95,95,100,100,100,
xylene (3)changes.
Fix testes in bouins for 24-48 hrs then transfer to 70%alcohol and process
from there. Stay consistent with fixation time.
Dog and primate brains process from 70% alcohol for 90minutes in all
reagents on different protocol.
Brains and Femoral head fixed in 30 % formalin. 24 hrs then tranfer to 10%
formalin. Bones get decal. Some labs fix everything in 10% Formalin.
All mice organs are processed on different protocol. 30 minutes in all
reagents start with formalin. Eyes start in 70%.
Stephanie D. Rivera
Safety Assessment Department
GlaxoSmithKline
709 Swedeland RD
King of Prussia, PA 19406
phone: 610-270-7340
fax: 610-270-7202
histoinfo@comcast.net
Sent by: histonet-bounces@lists.utsouthwestern.edu
18-Jun-2008 16:13
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Histonet@lists.utsouthwestern.edu
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Subject
[Histonet] Animal Tissue processing question
Dear Histonetters,
After doing human histology for 15 years I am excited to have the
opportunity to help start up a brand new research histology laboratory. It
is a very small lab so I will still have my day job. But it is all new and
different and a bit exciting. I would like to ask your help with the
processing times. I know animal tissue is dryer so I am thinking less time
in alcohol but am looking to those of you out there with the experience
for a place to start when it comes to VIP processing times. I know I will
be processing tissues from pigs and primates if that makes a difference.
Brand new VIP, brand new embedding center, brand new microtome. . . . I
can't tell you how exciting this is. Thanks for all your help.
Jennifer
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