Re: [Histonet] Androgen Receptor

From:stephanie.d.rivera@gsk.com



I have not used DAKO Androgen Antibody but I have tried the following:



I have run Affinity BioReagents using Ventana XT
DAB MAP KIT (1:50, 1:75, 1:100, 1:200) 60 minutes Primary, CC1 mild

Ultra MAP (1:500, 1:1000) 32 minutes, CC1 mild

Control tissue  RAT Repro (Prostate, Seminal Vesicles)

                ALSO 


Had success with Santa Cruz Polyclonal Rabbit Androgen(N20 clone) SC-816
Used AR Nuclear Decloaker PH9.0 in pressur cooker
Incubate overnight 4 degrees refrigerate
Rabbit envision-hrp(DAKO) for detection
Run @1ug/ml or 1:200
Control: Rat male repro(prostate) nuclear staining observed in epithelial 
cells of prostate.


 





Stephanie D. Rivera
Safety Assessment Department
GlaxoSmithKline
709 Swedeland RD
King of Prussia, PA 19406
phone: 610-270-7340
fax: 610-270-7202
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