RE: [Histonet] Gomeri Iron Stain

From:"Tony Henwood"



The following works well though it is a Perl's stain. I can't remember
the Gomori Iron stain. The old cranium is shrinking as I get older!!

It was developed for frozen sections (eg differentiating melanin from
iron pigment) but works quite well for paraffin sections.

1	Placed ethanol fixed frozen sections or hydrated paraffin
section in an the Perl's reagent in a plastic coplin jar (20ml each of
2% Potassium ferrocyanide and 2% HCl).
Microwave for 20 sec at 650watts.
2.	Leave sections in the hot solution for up to 3 minutes, remove
if the solution becomes cloudy.
3.	Rinse in water.
4.	Counterstain with eosin (as used for the frozen section H&E) or
a red nuclear stain (Nuclear Fast Red or Neutral red).
5.	Rinse in water, DC&M.

Reference:	Henwood (2002) "Microwave Perl's Stain for Urgent Frozen
Sections" Aust J Med Sc 23(2):68-69.

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
Histology Dept
Sent: Tuesday, 8 July 2008 1:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gomeri Iron Stain


We finally got a microwave for special stains.  Can you use a microwave
method to do Gomeri Iron Stain?  If so, would someone please share you
procedure with us?

Angie Barnett, HTL(ASCP) 
Grady Memorial Hospital 
Pathology Department 
405/224-2258 
histology@gradymem.org 
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