RE: [Histonet] Filter paper

From:"Lee & Peggy Wenk"



If memory serves me correctly, the lower the number, the thinner the paper.
And for most histology stains, thin works just fine, and is faster, too. 

But I'm curious - what staining procedure do you do that requires celestin
blue? We don't like making it up, so have either substituted an aluminum
hematoxylin (like Mayer or Gill), or an iron hematoxylin (like Weigert),
depending upon the stain or the type of nuclear contrast we want. And we
always have aluminum and iron hematoxylins around for lots of other stains,
so that saves us time from having to make up a third nuclear stain.

Peggy A. Wenk, HTL(ASCP)SLS
Beaumont Hospital
Royal Oak, MI 48073

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mylène de
Champlain
Sent: Wednesday, July 23, 2008 6:31 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Filter paper


Hi,
 
  To filter blue celestin, is what I have to use a filter paper number 1 or
4? Thank!
 
  Mylène de Champlain
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