RE: [Histonet] Animal Tissue processing question

From:"Burton, Lynn"

Here, at the Animal Disease Lab, in Galesburg, Illinois, we get tissues from every species one can imagine, and some you can't. We accept cases from area zoos, farms and veterinary clinics. These may be submissions of all organs or biopsies. With very few exceptions we use the following times on a Tissue Tek VIP processor:
1. formalin 30 minutes
2. formalin 30 minutes
3. 70% ETOH 30 minutes
4. 80% ETOH 30 minutes
5. 95% ETOH 30 minutes
6. 100 % ETOH 30 minutes
7. SAA
8. SAA
9. xylene or substitute, we've been using slide brite 45 minutes
10. SAA
11. paraffin 30 minutes
12. SAA
13. SAA
14. SAA
We process tissues daily using a delayed start program. All tissues trimmed that day are processed together. There are some tissues which are treated specially, such as lipomas, prior to processing. Most of our tissues are mailed or shipped to us in 10% NBF and so they have been fixed adequately prior to their arrival. I hope this is of help to you.
Lynn Burton
Animal Disease Lab
Galesburg, Il 


From: on behalf of
Sent: Wed 6/18/2008 3:13 PM
Subject: [Histonet] Animal Tissue processing question

Dear Histonetters,
After doing human histology for 15 years I am excited to have the opportunity to help start up a brand new research histology laboratory. It is a very small lab so I will still have my day job. But it is all new and different and a bit exciting. I would like to ask your help with the processing times. I know animal tissue is dryer so I am thinking less time in alcohol but am looking to those of you out there with the experience for a place to start when it comes to VIP processing times.  I know I will be processing tissues from pigs and primates if that makes a difference. Brand new VIP, brand new embedding center, brand new microtome. . . . I can't tell you how exciting this is. Thanks for all your help.
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