RE: [Histonet] Animal Tissue processing question

From:Jackie M O'Connor

We actually process beagle and rat tissue on the same program, mice are 
shorter (not their height - of course they are shorter - that's probably 
why they are meaner than rats - but I digress - - ).     Our tissues are 
absolutely perfect every time, not too hard, not too soft - -kinda like 
the 3 bears - - yeah, I'm going on vacation tomorrow, so my brain is 
already in vacation mode.   I'll be battling mosquitoes, pumas, and plague 
infested chipmunks in the Rocky Mountains.    Hawaii still sounded like a 
better idea.

Jackie O' 

"Rosa Fields"  
Sent by:
06/19/2008 09:30 AM

"Victor Tobias" , "Rene J Buesa" 

RE: [Histonet] Animal Tissue processing question

I would have to agree with Victor, also previously from a high volume vet 
diagnostic lab, as a matter of practicality all of our species were also 
grouped onto one processor.  It is very challenging to practically deal 
with everything one may see in a vet diagnostic lab! 

Rosa Fields, HT (ASCP)
Gastroenterology Specialties
Histology Supervisor
4545 R Street
Lincoln, NE  68503

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-----Original Message-----
[] On Behalf Of Victor 
Sent: Thursday, June 19, 2008 9:01 AM
To: Rene J Buesa
Subject: Re: [Histonet] Animal Tissue processing question

Having worked previously in a high volume vet diagnostic lab I somewhat 
disagree with Rene. For optimal processing I agree, but not for 
practicality. On a daily basis we would have every species under the sun 
(almost) and they got grouped together on one processor. Some days 
certain tissues might be a little more difficult, but not impossible.


Victor Tobias
Clinical Applications Analyst
University of Washington Medical Center
Dept of Pathology Room BB220
1959 NE Pacific
Seattle, WA 98195
206-598-7659 Fax
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Rene J Buesa wrote:
> You just mentioned 2 different species with peculiar characteristics. 
The difficulty with veterinary histology is that you will have to modify 
your procedures slightly to each, and you should never process tissues of 
different species in the same run.
>   From very "dry" mice tissues with less dehydration times, to pig 
tissues requiring more time in the antemedium because of the tisular fat 
(unless it is a boar) you will have to prepare modified protocol.
>   My advise, write down your protocols and the animals you used them 
with and evaluate the results in a manner that in some time you will have 
the protocols you need.
>   Sorry if this is not what you asked for, but, as I wrote you, there 
should be specific protocols.
>   René J.
> wrote:
>   Dear Histonetters,
> After doing human histology for 15 years I am excited to have the 
opportunity to help start up a brand new research histology laboratory. It 
is a very small lab so I will still have my day job. But it is all new and 
different and a bit exciting. I would like to ask your help with the 
processing times. I know animal tissue is dryer so I am thinking less time 
in alcohol but am looking to those of you out there with the experience 
for a place to start when it comes to VIP processing times. I know I will 
be processing tissues from pigs and primates if that makes a difference. 
Brand new VIP, brand new embedding center, brand new microtome. . . . I 
can't tell you how exciting this is. Thanks for all your help.
> Jennifer
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