2 questions for you all. What causes tissue sections after H&E stained fixed in formlain, to have nuclear bubbling? Is it under fixation, prolonged fixation? Also what is the best way to store for a short time, fresh, unfixed tissue either in a saline moistened gauze and refrigerated or just placing it in physiological saline at room temperature.  I am troubleshooting some problems. Karla Arringtonfreckles9660@yahoo.com      
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