I'm having problem to determine which positive controls are suitable to these marker for immunohistochemistry (IHC):PPARgamma,Flk-1, PKCalpha and eNOS.
I've already follow the antibody kit instruction provided by Santacruz Biotechnology Inc. and repeated 5x.but still cannot produce result.
these is the list of positive control for each antibody:
1)PPARgamma (sc-7273): Urothelial Carcinoma and Invasive Ductal Carcinoma (NOS)
2) Flk-1(sc-6251): Colon Carcinoma
3) PKCalpha(sc-8393): Colon Carcinoma
4) eNOS(sc-654): Serous Carcinoma@Ovary Carcinoma
Your kind response are very much appreciated!
Shazana Hilda Shamsuddin
MSc.of Molecular Pathology,
Dept. of Pathology,
School of Medical Sciences,
Universiti Sains Malaysia,
16150 Kubang Kerian,
Phone no: +6013-921 2002
Off. no: +609- 766 4440
Fax no: +609- 765 3370
Histonet mailing list