I was looking for advice on troubleshooting already cryoprotected tissue for
freezing artefact (FA). 

 

I have mice brains dissected out after cervical dislocation, postfixed in 4%
Paraformaldehyde/PBS overnight (4 degrees), cryoprotected in 30% sucrose for
~24 hours (4 degrees) and then placed in -80 freezer. This FA never was an
issue before and the animals are treatment free so anyone know why I would
be getting this now?

 

Regards,

 

Áine

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