[Histonet] anyone doing kidneys in general?

From:Jennifer Sipes

Hi everyone!
We're looking at the proximal tubules in mice and we're finding that  
they are either collapsed or whole looking but the edges are  
crinkled.  I'm perfusing the kidney, but I'm wondering if anyone had  
any suggestions on how to get nice whole looking proximal tubules?   
Should I maybe perfuse more to ensure that they're opening up for us  
or is there another method?
Please let me know and thanks in advance for all your help!
Jennifer K. Sipes, ALAT
Sr. Laboratory Technician
Johns Hopkins University
Ross 929 and Ross 933
720 Rutland Avenue
Baltimore, MD  21205
phone:  410-614-0131
fax:         410-955-9677
cell:        443-631-6361
e-mail:  jsipes1@jhmi.edu

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