[Histonet] Vector Labs Nova HRP--Red Substrate: Not Really EtOH compatible?

From:JR R




The Product information says: 

"NovaREDô substrate may be
dehydrated in ethanol and cleared in organic solvents such as xylene
and permanently mounted in a non-aqueous mounting medium such as
VectaMount"


But I have done two stains now where I initially had intense, specific staining at first--But after my slides come out of the EtOH/Xylene for coverslipping, the staining has vanished.

This is getting to be expensive!

Has anyone else ever had this problem with Nova Red?  Any ideas?

Jerry Ricks
Research Scientist
University of Washington
Department of Pathology




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