I use the VZV from Novacastra.
It works best for me when made up fresh each run.
If you have run the antibody fresh, then try using fresh antibody and
cut "fresh" sections from the paraffin block for control tissue.
I run it 1:50 with Biocare Mach 3. If you are using and ABC kit of
anykind you may need to go even stronger, 1:10.
Try one slide with pepsin and one with out pepsin.
We get best results with no HIER or enzyme pretreatment.
Hope this helps.
Becky Orr CLA,HT(ASCP)QIHC
Evanston Northwestern Healthcare
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