[Histonet] Single-cell susension from tissue

From:hyters@onid.orst.edu



hello, having trouble fully dissociating dermal tissue to use in a  
cell count on a hemacytometer.  Can I vortex cells in suspension or  
should I just keep them in the enzyme bath a bit longer? (worried  
about surface markers for FACS)

thanks for any help,
stephen

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